This book constitutes the refereed proceedings of the 16th EUNICE/IFIP TC 6.6 Workshop on Networked Services and Applications, EUNICE 2010, held in Trondheim, Norway, in June 2010. The 24 revised full papers presented together with 6 posters were carefully reviewed and selected from numerous submissions. The papers are organized in topical sections on admission control and networking; service mobility; peer-to-peer and virtualization; security; congestion control; monitoring and filtering; dependability; and adaptation and reconfiguration.