This volume constitutes the refereed proceedings of the 2ndInternational Conference on Advanced Science and Technology, AST 2010, held in Miyazaki, Japan, in June 2010.The 16 revised full papers presented in this volume were carefullyreviewed and selected from 220 submissions. The papers address currentissues in computer science and its applications, software engineeringand its applications, bio-science and bio-technology, u- and e-service, science and technology, database theory and application, control andautomation, signal processing, image processing and pattern recognition, as well as grid and distributed computing.