This book constitutes the refereed proceedings of the 12th Ibero-American Conference on Artificial Intelligence, IBERAMIA 2010, held in Bah a Blanca, Argentina, in November 2010. The 61 papers presented were carefully reviewed and selected from 148 submissions. The papers are organized in topical sections on artificial intelligence in education, cognitive modeling and human reasoning, constraint satisfaction, evolutionary computation, information, integration and extraction, knowledge acquisition and ontologies, knowledge representation and reasoning, machine learning and data mining, multiagent systems, natural language processing, neural networks, planning and scheduling, probabilistic reasoning, search, and semantic web.